Mona El Shabrawy
National Research Centre, Egypt
Title: More perception into the variance of coagulase positive Staphylococci with particular consideration to coagulase gene polymorphism
Biography
Biography: Mona El Shabrawy
Abstract
Two hundred and twenty six samples were collected from human, dog, cat and poultry and examined for the existence of coagulase positive staphylococci. The conventional slide method identified 32 staphylococcal isolates as coagulase positive whereas this number was reduced by tube method to be 20 isolates. Based on Voges-Proskauer and Polymyxin B resistance tests, the isolates were differentiated into 16 S. aurues and two S. intermedius. The PCR amplification of thermonuclease and coagulase genes was conducted on S. aurues isolates, and then the coagulase positive products were put through restriction digestion using RFLP with AluI. Four distinct profiles (I, II, III &IV) were obtained. The coa gene was sequenced and exhibited a major genotype of S. aureus clone despite the different species origins supposing a common ancestor. The achieved data put this study in scene when proposed control strategies of staphylococcal infection as the issues in the regard.