Idress Attitalla
Omar Al-Mukhtar University, Libya
Title: Candida species-specific Th1, Th2, and Th17 cell immune response in human peripheral blood mononuclear cells (PBMC)
Biography
Biography: Idress Attitalla
Abstract
Background: Although non-albicans Candida (NAC) is the source of invasive infections, little is known about the immune responses persuaded by these species. Understanding the host defense mechanisms of each Candida species is vital in the development of antifungal vaccines and drugs. Here we demonstrate of the differential regulation of Th1, Th2, and Th17 during Candida species in vitro.
Methods: We compared the cytokine production capacity of human peripheral blood mononuclear cells (PBMC) upon stimulation with different Candida species. We measured secreted cytokines by ELISA and checked the functional profile of T cell responses at the single-cell level by a multi-color flow cytometry technique.
Results: Despite the differential expression of cytokines against Candida species, a significant difference was observed in the levels of IFN-γ, TNF-α, IL-10, IL-12p40, and IL-between Candida. species. Baseline levels of anti-inflammatory cytokines (IL-4 and IL-10) were significantly lower than pro-inflammatory cytokines in all stimuli. Although the percentages of CD4+ and CD8+ expressing Th1, Th2 and Th17 cytokines were higher in stimulated PBMC, none of the Candida species showed significant differences. Levels of secreted IL-17A and IL-23 were consistently lower in Candida species regardless of the stimulus used.
Conclusions: The current results indicate that C. albicans induces a strong Th1-biased response and C. glabrata induces strong Th2 cellular responses. It improves our knowledge of the differential immune responses induced by Candida species and may help develop immune therapeutics.