Microbial Interactions & Microbial Ecology

Biography

Biography: Adriane Wendland

Abstract

Common bean (Phaseolus vulgaris L.) belongs to the order Rosales, family Fabaceae (Leguminosae). Brazil stands out worldwide among the largest producers and consumers of beans, which are grown throughout the territory, in different environmental and national conditions. However, illness is one of the main factors of bean fever. Therefore, the objective of this study was to characterize and identify Pseudomonas isolates and verify the pathogenicity in the common bean crop. The method used for the biochemical characterization of the isolates were the LOPAT tests, and the method used for the molecular identification were the conventional PCR technique for the identification P. fluorescens using specific primers. To evaluate the pathogenicity of Pseudomonas isolates in common bean cultivars, plants of cultivars BRS Embaixador, BRS Esplendor, BRS Esteio, BSR FS 305 e BRS Marte were submitted to inoculation tests in a controlled environment and in a greenhouse using the isolates BRM 65465 and BRM 65466, by the multiple needle technique for perforating the leaf blade. From 3 to 14 days after inoculation, they were evaluated for the incidence of symptoms from the point of inoculation. The biochemical and molecular tests indicate that the isolates BRM 65465 and BRM 65466 are Pseudomonas fluorescens. The symptoms caused by the isolates BRM 65465 and BRM 65466 in the pathogenicity tests indicate that they are pathogenic for the common bean crop, where, in a greenhouse, under conditions of higher temperatures, the symptoms were shown more severe, compared to a controlled environment with milder temperatures.