The gut microbiota is constituted by complex microbial communities, that is, by microorganisms of different species that interact with each other intra- and interspecifically, as well as with their environment [1]. This ecosystem can easily be altered by several factors, among which are the flu-like processes caused by viruses of the Orthomyxoviridae family [2,3].rnThe causative agent of influenza (Myxovirus influenzae) is responsible for both the outbreaks of seasonal flu and major pandemics throughout history [4]. Often, these conditions are complicated by the involvement of secondary bacterial infections. The increasing level of resistance to antibiotics may be responsible for the death of 10 million people a year, in 2050 [1,5]. This fact increases the scientific interest to know the factors that can affect the gut microbiota and its repercussions.rnThe purpose of this work is to analyze the possible impact of the IAV virus on the functional status and phenotypic expression profile of the antibiotic resistance of the mice gut microbiota. To this effect, the biotechnological adequacy of techniques traditionally used for the evaluation of the antibiogram of bacterial populations was proposed, for the study of the behavior of the intestinal microbial community (microbiota) against antibiotics (cenoantibiogram). For this, mice were inoculated with influenza virus in PBS (IAV), controls inoculated with PBS buffer (Mock) and controls without virus or PBS (baseline).rnResults of the statistical analysis (Random forest and Wilcoxon indices) show that the gut microbiota is significantly modified after the infection of the influenza virus, increasing its resistance to the antibiotics ampicillin and cefepime. The possible finding of factors that allow the acquisition and permanence of bacterial resistance, as well as its correlation with the influenza virus, could be a finding of great epidemiological relevancern
