Guliko Dvali has completed her PhD in Microbiology from Ivane Javajishvili Tbilisi State University. She is the Head of mcrobilogy research group in Georgian Technical University, Biotechnology Center. She has published more than 20 papers in reputed journals and has been a great experience in plant miocrobiology field.
Study of the biopreparation influence on pathogens, which causes the disease of soil micro-flora and plant, is one of the main issues for environmental recovery and ecologically clean product. The purpose of the research was to study the influence of the pathogen Fusarium causing the disease of tomato root and the study of the influence of biopreparation “Phytocatena” (Pseudomonas fluorenscens), produced by the Plant Biological protection Center in Georgia, on the pathogen. The tomato variety “Slivka” has been studied for the experiment. Microbiological analysis was conducted by M. Wozniakovskaya’s method. The cultivation of microbes was occurred in potato, Chapek, Suslo’s food areas, with various suspensions (1:10, 1:100, 1:1000). The total quantity of pathogenic fungi was estimated by thousands of 1 gram on dry ground. As a result of research, the total quantity of pathogenic fungi in the untreatment soil was 400 thousand. The dominant pathogen was Fusarium (205 thousand). Soil and tomato root system was processed by the 2% solution of bio-preparation "Phytocatena" before the planting, and then by 2-4 times with 15-20 days interval. It has been determined, that the total quantity of pathogenic fungi in untreatment soil was 261 thousand (140 thousand on plant’s roots,121 thousand in the rhizosphere). The total quantity of Fusarium was 120 thousand (65 thousand on plant’s roots, 55 thousand in rhizosphere). From samples collected in the phase of tomato blossom, it has been found, that after the treat by the biopreparation "Phytocatena", the total quantity of Fusarium was significantly reduced-20 thousand (11 thousand on plant’s roots, 9 thousand in the rhizosphere). The total quantity of pathogenic fungi was also reduced by 49 thousand (25 thousand on the plant’s root, 24,000 in the rhizosphere).
Tamar Shamatava has completed her PhD at St. Andrew the First Called Georgian University of the Patriarchate of Georgia from 2010-2015. She is the Senior Scientist at Georgian Technical University Biotechnology Center. She has published more than 17 papers in reputed journals. She has a great experience in agriculture and biotechnology field
Background &Aims: The loss causing of pathogenic microbiological diseases during storage has a high economic impact. The research aim was to study effect of eucalyptus extract with combination calcium chloride on microflora of grapevine during storage. Materials & Methods: Two grapevine varieties were selected for study: Alphonse Levallée and Italia. Two combinations of eucalyptus extract and calcium chloride were selected for experiment: I.1 % CaCl2 and 2% eucalyptus extract II. 2% CaCl2 and 1% eucalyptus extract III. Control-untreatment grapevine. Treatment and control both were stored storage refrigerator -POLAIR Standard (temperature-0-10C, humidity- 85-90%). Findings: Pathogenic clear cultures were extracted from infected grapevine during storage (60-120 day). It was revealed that Botrytis cinerea and Penicilium expansum were two major infected agent which causing microbiological disease of grapevine varieties Alphonso levalee and Italia. Characterization and identification of fungi carried out using 40X-2500X professional infinity Trinocular Compoud Microscope (SKU:T690C). As a result showed the loss caused from phytopatohenic fungi were different-Control for grapevine varieties Italia with Botrytis cinerea was-55.3% and Penicilium expansum-37.6%. For Alphonso Levalée by Botrytis cinerea-54.1% and Penicilium expansum-35.2%. The best result for grapevine varieties Italia was showed 2% CaCl2 and 1% eucalyptus extract, in this case loss causing by Botrytis cinerea-42. 8%, and Penicilium expansum- 32.4%, but inhibition effect caused from Botrytis cinerea for Alphonso Levalee was-45.1% and Penicilium expansum-30.4%. Conclusions: Thus, the combination of 2% CaCl2 and 1% eucalyptus extract had inhibition influence on developments of Botrytis cinerea and Penicilium expansum, especially on Botrytis cinerea.